Journal: Clinical Cancer Research

Article Title: T-Cell Receptor Repertoire Sequencing in the Era of Cancer Immunotherapy

doi: 10.1158/1078-0432.CCR-22-2469

Figure Lengend Snippet: TCR sequencing platforms.

Article Snippet: AmpliSeq TCRb Panel , Illumina , Illumina MiniSeq, MiSeq, NextSeq 1000, NextSeq 2000, NextSeq 550 , Bulk , Yes , Multiplex PCR , RNA , CDR1 CDR2 CDR3 , TCRβ , Human , .

Techniques: Sequencing, Multiplex Assay, Generated, RNA Extraction, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Two decades of CRISPR-Cas9 adoption and success .

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Successful application of CRISPR-Cas9 in different plant species.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR, Modification, Gene Knockout, Cell Surface Receptor Assay, Activity Assay, Expressing

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: How CRISPR-Cas9 perform genome editing. Cas9 induce double stranded breaks (DSBs) at particular site. The resulting DSB is then repaired by one of these two general repair pathways, e.g., by Non-homologous end joining (NHEJ) or by Homology directed repair (HDR). (A) The NHEJ repair pathway frequently results in small nucleotide insertions or deletions (InDels) at the DSB site. This may result in gene knock out or gene insertion. (B) HDR can be used to generate precise nucleotide modifications (also called gene “edits”) ranging from a single nucleotide change to large insertions.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR, Non-Homologous End Joining, Knock-Out

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Tabular presentation of comparative attributes of plant genome editing techniques.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: Zinc-Fingers, TALENs, Sequencing, Clone Assay, Produced, In Vitro, Methylation, Multiplexing, CRISPR, Mutagenesis

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Technical limitations in CRISPR-Cas9 application and their effects.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR, Concentration Assay, Expressing, Sequencing, DNA Methylation Assay, Modification, Protein Binding

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: List of promoters and gene(s) targeted through CRISPR-Cas9 system in different plants.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Different plasmids with their genes, vectors, and promoters used in CRISPR-Cas9 technique.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR, Plasmid Preparation, Expressing, Sequencing

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Diagrammatic illustration of live-cell DNA labeling by using CRISPR-Cas9 system .

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: DNA Labeling, CRISPR

Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Specific commercial products and services available to the researchers to implement CRISPR technology.

Article Snippet: Bio Labs England , Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix , Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) , EnGe Cas9 Nuclease , EnGen Mutation Detection Kit.

Techniques: CRISPR, Genome Wide, Clone Assay, Stable Transfection, Selection, Transfection, Plasmid Preparation, Mutagenesis, Expressing, Negative Control, Positive Control, Construct, Knock-In, Multiplex Assay, Amplification, Sequencing